Reverse Transcription and qPCR Amplification
Corresponding Organization :
Other organizations : Medical University of Graz, Heinrich Heine University Düsseldorf, Deutsches Diabetes-Zentrum e.V., German Center for Diabetes Research, ETH Zurich, Utrecht University, German Institute of Human Nutrition, Humboldt-Universität zu Berlin, Charité - Universitätsmedizin Berlin, Freie Universität Berlin, Stanford University, California Institute for Regenerative Medicine, University Hospital Leipzig, Leipzig University, Heidelberg University, University Hospital Heidelberg, BioTechMed-Graz
Variable analysis
- Reverse transcription using High-Capacity cDNA Reverse Transcription Kit
- CDNA amplification using Blue SybrGreen qPCR Mastermix
- CDNA levels
- Primer sequences (provided in Supplementary Table 1)
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