Immunofluorescence Imaging of Transfected Cells

Immunofluorescence was performed as previously described (Sui et al., 2021 (link)). HEK293T or HepG2 cells cultured on 12-mm coverslips were transfected with the indicated plasmids. After 24 h, cells were fixed with 4% paraformaldehyde and permeated with 0.5% Triton X-100. After cells were washed with PBST, they were blocked in 1% BSA and stained with primary antibodies, followed by staining with CoraLite 594 or 488-conjugated IgG secondary antibodies. Nuclei were stained with DAPI (Yesen Biotechnology, Shanghai, China). Fluorescence images were obtained and analyzed using a confocal microscope (FV3000, OLYMPUS).