Confocal Microscopy Imaging Protocol

All images were acquired with an LSM700 AxioObserver laser scanning confocal microscope equipped with a plan Apochromat 40x/1.3 Oil DIC M27 (Zeiss, Oberkochen, Germany) objective, using a gallium arsenide phosphide photomultiplier tube (GaAsp-PMT) detector controlled by Zen black software (version 8.0.7.273, Zeiss, Oberkochen, Germany). After acquisition, images were processed using the Fiji ImageJ2 software (National Institutes of Health, Bethesda, MD, USA).

Free full text: Click here

Serafini S., Ferretti G., Monterosso P., Angiolillo A., Di Costanzo A, & Matrone C. (2024). TNF-α Levels Are Increased in Patients with Subjective Cognitive Impairment and Are Negatively Correlated with β Amyloid-42. Antioxidants, 13(2), 216.

Publication 2024

LSM700 AxioObserver Zen black software

Corresponding Organization : University of Molise

Top 5 similar protocols

Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Not explicitly mentioned

control variables

Microscope: LSM700 AxioObserver laser scanning confocal microscope
Objective: plan Apochromat 40x/1.3 Oil DIC M27 (Zeiss, Oberkochen, Germany)
Detector: gallium arsenide phosphide photomultiplier tube (GaAsp-PMT)
Software: Zen black software (version 8.0.7.273, Zeiss, Oberkochen, Germany)
Image processing: Fiji ImageJ2 software (National Institutes of Health, Bethesda, MD, USA)

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!