Visualizing Inflammatory Response in hPdLFs

To visualize the inflammatory response of hPdLFs, the activation of THP1 monocytic cells was analyzed as previously described [40 (link)]. CMFDA (Thermo Fisher Scientific, Carlsbad, CA, USA)-stained THP1 cells were added to each well of stimulated hPdLFs. After the removal of non-adherent cells, the remaining adherent cells were fixed for 10 min in 4% paraformaldehyde (PFA). DAPI (1:10,000 in PBS) staining was used to visualize cell nuclei. Membrane cut-outs were embedded with Mowiol^® 4-88 (Carl Roth, Karlsruhe, Germany) on glass object slides.

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Steinmetz J., Stemmler A., Hennig C.L., Symmank J, & Jacobs C. (2024). GDF15 Contributes to the Regulation of the Mechanosensitive Responses of PdL Fibroblasts through the Modulation of IL-37. Dentistry Journal, 12(2), 39.

Publication 2024

CMFDA Mowiol® 4-88

Corresponding Organization : Jena University Hospital

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Variable analysis

independent variables

Stimulation of hPdLFs

dependent variables

Activation of THP1 monocytic cells

control variables

Removal of non-adherent cells
Fixation of cells for 10 min in 4% paraformaldehyde (PFA)
DAPI (1:10,000 in PBS) staining to visualize cell nuclei
Embedding of membrane cut-outs with Mowiol® 4-88

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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