Mice adipose tissue and liver paraffin-embedded sections were cut at 4μm and stained with hematoxylin and eosin (H&E) as well as Masson’s Trichrome C staining, performed by the University of Texas Southwestern Medical Center Histology Core. Images (100× or 200× magnification) were recorded by the FSX100 Inverted Microscope (Olympus, Waltham, MA, USA), and representative histological images were shown.
For adipocyte size quantification, bright-field H&E staining images were taken through a Keyence BZ-X710 microscope (Keyence, Itasca, IL, USA). Adipocyte size analysis was conducted according to previously validated protocols42 (link). Incorporated Keyence BZ-X Analyzer software was utilized for analyzing and calculating the area for each adipocyte. At least 600 adipocytes were quantified for each individual mouse.
For adipocyte size quantification, bright-field H&E staining images were taken through a Keyence BZ-X710 microscope (Keyence, Itasca, IL, USA). Adipocyte size analysis was conducted according to previously validated protocols42 (link). Incorporated Keyence BZ-X Analyzer software was utilized for analyzing and calculating the area for each adipocyte. At least 600 adipocytes were quantified for each individual mouse.
