Tissue temperature in the interscapular BAT and the skeletal muscle was recorded using the type T thermocouple probes and recorded by using the TC-2000 Meter (Sable Systems International), according to our protocol3 (link). Core body temperature was monitored using a TH-5 thermometer (Physitemp) by recording the rectal temperature of the mice. For core-body temperature recording experiment in Fig. 5i , both control (Ppargflox/flox) and PpargMyoD KO (Myod-CreERT2;Ppargflox/flox) mice were pre-treated with β-blocker and tamoxifen at room temperature for 5 days and acclimated to 15°C for additional 5 days. Wild-type mice did not receive pre-β-blocker-treatment. Subsequently, all the mice were acutely treated with β-blocker and exposed to 10°C.
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Measuring Tissue Temperature in Mice
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Variable analysis
independent variables
- Presence or absence of β-blocker pre-treatment
- Presence or absence of tamoxifen pre-treatment
- Acclimation to 15°C for 5 days
- Acute β-blocker treatment and exposure to 10°C
- Tissue temperature in the interscapular BAT
- Tissue temperature in the skeletal muscle
- Core body temperature
- Thermocouple probe type (type T)
- TC-2000 Meter (Sable Systems International) for temperature recording
- TH-5 thermometer (Physitemp) for core body temperature recording
- Rectal temperature for core body temperature measurement
- Experimental conditions for control (Pparg^flox/flox) and knockout (Pparg^MyoD KO) mice
- Control (Pparg^flox/flox) mice
- Wild-type mice without pre-β-blocker-treatment
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