Collection of plaque samples was done from patients with inflammation in periodontal cells. The plaque samples were collected from patients diagnosed as generalized periodontitis stage III grade B. The diagnosis was based on the distribution and severity of periodontal attachment loss in relation to teeth present in the oral cavity [23 (link)]. Plaque samples were accumulated by infusing Gracey-curette (Hu-Friedy, Chicago, IL, USA). The plaque samples were collected as the curette touched the base of the periodontitis region without any damage to adjacent tissues. The plaque samples were moved to an anaerobic transport media, Sodium thioglycolate (Sigma-Aldrich, Dermstadt, Germany).
Selective media was used for the isolation of P. gingivalis, T. denticola, A. actinomycetemcomitans, and T. forsythia [24 (link)], incubated anaerobically with nitrogen content of 80%, hydrogen content 10%, and carbon dioxide content 10% for a period of 3 to 7 days at a temperature of 37 °C. Further, the growth of the desired bacteria on the particular media was recognized based on morphology of the colony, and species identification was done by biochemical tests.
Selective media was used for the isolation of P. gingivalis, T. denticola, A. actinomycetemcomitans, and T. forsythia [24 (link)], incubated anaerobically with nitrogen content of 80%, hydrogen content 10%, and carbon dioxide content 10% for a period of 3 to 7 days at a temperature of 37 °C. Further, the growth of the desired bacteria on the particular media was recognized based on morphology of the colony, and species identification was done by biochemical tests.
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