Derivatization of estrogens and estrogen metabolites were achieved with dimethylaminoaphthalene Dansyl Chloride ≥ 99.0% HPLC sourced from Sigma Aldrich (St. Louis, MO). Dansyl Chloride-D6 was sourced from C/D/N Isotopes, Inc. (Pointe-Claire, Quebec, Canada) and used to generate in-house internal standards for all 15 estrogens and estrogen metabolites. β-glucuronidase/sulfatase from Helix pomatia was obtained from Millipore Sigma (St. Louis, MO). Glacial acetic acid (Certified ACS), L-ascorbic acid (Certified ACS), acetone (HPLC grade), acetonitrile (Optima HPLC grade), and methanol (Optima HPLC grade) were (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
purchased from Fisher Chemical (Pittsburgh, PA). Sodium bicarbonate is purchased from Sigma Life Science (S5761). Sodium carbonate enzyme grade (Anhydrous) was purchased from Fisher Scientific (Waltham, MA). Sodium acetate (for HPLC) was acquired from Honeywell / Fluka (Morris Plains, NJ). Supported liquid extraction 400uL cartridges, 1cc cartridges, and 2cc cartridges were acquired from Biotage® (Charlotte, NC). Mass spectrometry vials 0.3mL and 1mL were purchased from Thermo Scientific (Waltham, MA).
purchased from Fisher Chemical (Pittsburgh, PA). Sodium bicarbonate is purchased from Sigma Life Science (S5761). Sodium carbonate enzyme grade (Anhydrous) was purchased from Fisher Scientific (Waltham, MA). Sodium acetate (for HPLC) was acquired from Honeywell / Fluka (Morris Plains, NJ). Supported liquid extraction 400uL cartridges, 1cc cartridges, and 2cc cartridges were acquired from Biotage® (Charlotte, NC). Mass spectrometry vials 0.3mL and 1mL were purchased from Thermo Scientific (Waltham, MA).
