After the Resazurin measurement, cells were fixed with 99% ethanol at −20 °C for at least 1 h. After washing once with tap water, the wells were incubated with 0.4% SRB solution (Sigma-Aldrich, MO, USA) for 30 min. Cells were washed 3–4 times with 1% acetic acid. Staining was resolved with 10 mM of unbuffered Tris solution and absorbance was measured at 565 nm and 690 nm with the Omega plate reader. The absorbance values at 690 nm (impurities) were subtracted from the values at 565 nm [50 (link)].
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