qRT-PCR Gene Expression Analysis

The isolated RNA was reverse transcribed into cDNA by an M-MuLV cDNA Synthesis Kit (Sangon Biotech). The primers of this study for qRT-PCR were designed by Primer Premier 5.0 software to verify the relative expression level of randomly selected genes with the 18S gene as an internal control. The relative expression of these randomly selected genes was calculated with the 2^−△△Ct method. The PCR conditions were set as follows: predenaturation at 95°C for 10 min and 40 cycles of 95°C for 10 s and 60°C for 30 s (Wu et al., 2021 (link)).

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Wu M., Liu H., Zhang Y., Li B., Zhu T, & Sun M. (2023). Physiology and transcriptome analysis of the response mechanism of Solidago canadensis to the nitrogen addition environment. Frontiers in Plant Science, 14, 1005023.

Publication 2023

M-MuLV cDNA Synthesis Kit

Cdna Expression genes Gene control M mulv Primer Synthesis

Corresponding Organization : Henan University of Urban Construction

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Relative expression level of randomly selected genes

control variables

18S gene used as an internal control

controls

No positive or negative controls explicitly mentioned

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