In accordance with the Declaration of Helsinki Principles, ethical approval was
granted by the Institute of Ethical Committee of Nanfang Hospital, Southern
Medical School (approval number: NFEC-2021-265). HPKs were derived from the
scalp tissues of 20 to 30-year-old men or women after dermatoplasty. Personal
identity information was not obtained. To facilitate enzymatic lysis, the skin
was cut into pieces and incubated overnight in dispase II (Roche, Mannheim,
Germany) at 4°C. The next day, the epidermis was digested with 0.25%
trypsin-EDTA solution (Coolaber, China) for 20 min at 37°C, as previously
described.19 (link) The isolated keratinocytes were cultured in defined
keratinocyte-SFM (ScienCell, 2111, USA) plus 20 μM Y-27632 (APExBIO, B1293,
Houston, USA),20 and the medium was changed every 3 days.
granted by the Institute of Ethical Committee of Nanfang Hospital, Southern
Medical School (approval number: NFEC-2021-265). HPKs were derived from the
scalp tissues of 20 to 30-year-old men or women after dermatoplasty. Personal
identity information was not obtained. To facilitate enzymatic lysis, the skin
was cut into pieces and incubated overnight in dispase II (Roche, Mannheim,
Germany) at 4°C. The next day, the epidermis was digested with 0.25%
trypsin-EDTA solution (Coolaber, China) for 20 min at 37°C, as previously
described.19 (link) The isolated keratinocytes were cultured in defined
keratinocyte-SFM (ScienCell, 2111, USA) plus 20 μM Y-27632 (APExBIO, B1293,
Houston, USA),20 and the medium was changed every 3 days.
