NLRP3 knockout (NLRP3–/–) or wild-type (WT) (C57BL/6) mice in the model group received 10 mg/kg of 200 μL LPS (E. coli O111:B4; Sigma-Aldrich) via intraperitoneal injection to induce ALI.9 (link) The control mice were administered with 200 μL PBS. After 6 or 12 hours, mice were anesthetized. Retro-orbital blood and BALF were collected, and the left lungs were resected. Samples were stored for biochemical, protein or nucleus assays. For histological analysis, blood cells were washed out from the right ventricle by PBS perfusion before lung harvest. The Animal Care and Use Committee of the Third Affiliated Hospital of Sun Yat-sen University approved the experimental procedure in this study (no. 00129136) according to the NIH Guide for the Care and Use of Laboratory Animals.
Protocol detail
Lipopolysaccharide-Induced Acute Lung Injury
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Animal
Assays
Blood
Blood cells
C57bl mice
E coli
Intraperitoneal injection
Laboratory animals
Lung
Mice
Nucleus
Perfusion
Protein
Right ventricle
Corresponding Organization :
Other organizations : Sun Yat-sen University, Third Affiliated Hospital of Sun Yat-sen University, The First Affiliated Hospital, Sun Yat-sen University
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Variable analysis
independent variables
- NLRP3 knockout (NLRP3^{–/–}) or wild-type (WT) (C57BL/6) mice in the model group
- Administration of 10 mg/kg of 200 μL LPS (E. coli O111:B4; Sigma-Aldrich) via intraperitoneal injection
- Biochemical, protein or nucleus assays on collected samples (retro-orbital blood, BALF, and left lungs)
- Histological analysis of the right lung
- Control mice administered with 200 μL PBS
- Blood cells washed out from the right ventricle by PBS perfusion before lung harvest
- Positive control: NLRP3^{–/–} or WT (C57BL/6) mice received LPS to induce ALI
- Negative control: Control mice received PBS
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