Escherichia coli XL10-Gold and BL21(DE3) Gold cells were purchased from Stratagene. EZ-Run protein ladder was purchased from Fisher Bioreagents and Ni2+-nitrilotriacetate (Ni-NTA) resin from Qiagen. IANBD, Click-iT® Protein Reaction Buffer kit and iodoacetamide alkyne were obtained from Life Technologies. BADAN, oxazine derivatives (Blue Oxazine, Red Oxazine and Nile Blue) and Chromis dyes (Chromis 630 and 678) were purchased from Eurogentec, Chromeon and Cyanagen, respectively. The merocyanine dyes (Mero 53, 62 and 87) were synthesized as previously described [10 (link)–12 (link)]. Texas Red–dextran was obtained from Life Technologies.
Protocol detail
Fluorescent Protein Labeling Strategies
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EZ-Run protein ladder
Ni2+-nitrilotriacetate (Ni-NTA) resin
IANBD
Click-iT® Protein Reaction Buffer kit
iodoacetamide alkyne
Blue Oxazine
Red Oxazine
Nile Blue
Chromis dyes (Chromis 630 and 678)
Texas Red–dextran
Alkyne
Badan
Buffer
Cells
Derivatives
Dextran
Dyes
Escherichia coli
Gold
Ianbd
Iodoacetamide
Merocyanine dyes
Nile blue
Oxazine
Protein
Resin
Corresponding Organization :
Other organizations : St George's, University of London, King's College London, Guy's Hospital, University of North Carolina at Chapel Hill
Top 5 similar protocols
Variable analysis
independent variables
- Cell types (Escherichia coli XL10-Gold and BL21(DE3) Gold cells)
- Not explicitly mentioned
- EZ-Run protein ladder
- Ni2+-nitrilotriacetate (Ni-NTA) resin
- IANBD
- Click-iT® Protein Reaction Buffer kit
- Iodoacetamide alkyne
- BADAN
- Oxazine derivatives (Blue Oxazine, Red Oxazine and Nile Blue)
- Chromis dyes (Chromis 630 and 678)
- Merocyanine dyes (Mero 53, 62 and 87)
- Texas Red–dextran
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