Laboratory personnel were masked to the identity of the samples. Samples were prepared for metagenomic deep sequencing, as previously described.13 (link) Briefly, total RNA was extracted from 100 µL aqueous fluid samples using the Zymo quick-RNA microprep kit (Zymo Research, Irvine, CA) per the manufacturer's instructions. Sequencing libraries were prepared using the NEBNext RNA Ultra II kit (New England Biolabs, Ipswich, MA) and then amplified with 21 cycles. Libraries were sequenced on the NovaSeq 6000 using two technical replicates.
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Metagenomic Sequencing of Aqueous Fluid Samples
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Corresponding Organization : San Francisco General Hospital
Other organizations : San Francisco Foundation
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Variable analysis
independent variables
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- Laboratory personnel were masked to the identity of the samples
- No positive or negative controls were explicitly mentioned in the provided information.
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