Evolutionary Analysis of Bird Fecal Parvoviruses

To investigate the evolutionary relationship of bird fecal parvovirus to other members of the family Parvoviridae, translated sequences from the coding region NS of wild bird fecal parvoviruses and reference sequences in GenBank were aligned using MUSCLE in MEGA v10.2.2 with default settings. Bayesian inference trees were then constructed using MrBayes v3.2 (RRID:SCR_012067) [46 (link)]. During MrBayes analysis, we set “prset aamodelpr = mixed” for the phylogenetic analysis using amino acid sequences, which allows the program to utilize the 10 built-in amino acid models. The Markov chain was run for a maximum of 1 million generations, in which every 50 generations were sampled and the first 25% of Markov chain Monte Carlo samples were discarded as burn-in.