Investigating Itaconate Effects on Allergic Airway Inflammation

C57BL/6 and Irg1^−/− mice were sensitized by two intraperitoneal injections of 10 µg OVA (Sigma, St. Louis, MO, USA) complexed with 1 mg potassium aluminum sulfate (Sangon Biotech, Shanghai, China) or saline on day 0 and day 7, then challenged with 1% aerosolized OVA or saline for 30 min per day on days 14–20. Additionally, mice received intraperitoneal injections of itaconate (30 mg/kg, 100 mg/kg, 150 mg/kg, Sigma) or MitoTEMPO (5 mg/kg, Sigma) before each OVA challenge. All mice were sacrificed on day 21; after collecting serum, the left lung was subjected to a bronchoalveolar lavage and subsequent differential cell counting and ELISA analysis, while the right lung of each mouse was used for a histopathological analysis, immunohistochemistry and further Western blotting assays.