Six-week-old male SHRs (130–150 g) and five-week-old male Wistar Kyoto (WKY) rats (130–150 g) were obtained from Charles River, Inc (Beijing, China, SCXK (Jing) 2016-0006). All animals were housed under controlled light (12 h/12 h light–dark cycle), humidity 55% ± 5% and temperature 22.1 °C ± 0.5 °C with free access to water and standard chow. There was one week of adaptive feeding before the formal experiment. All experimental procedures were approved by the Biological and Medical Ethics Committee, Minzu University of China (approval No. ECMU201807).
A total of 32 SHRs were divided into 4 groups: SHR-M (double-distilled water), SHR-Losartan (SHR-Los, 20 mg/kg/day) [79 (link)], SHR-Aspirin (SHR-Asp, 10 mg/kg/day) [80 (link)], and SHR-Atorvastatin (SHR-Ato, 10 mg/kg/day) [81 (link)]. All these drugs were dispersed in double-distilled water and were administrated daily to SHRs via oral gavage for 16 weeks. WKY rats were treated with double-distilled water as normotensive controls.
A total of 32 SHRs were divided into 4 groups: SHR-M (double-distilled water), SHR-Losartan (SHR-Los, 20 mg/kg/day) [79 (link)], SHR-Aspirin (SHR-Asp, 10 mg/kg/day) [80 (link)], and SHR-Atorvastatin (SHR-Ato, 10 mg/kg/day) [81 (link)]. All these drugs were dispersed in double-distilled water and were administrated daily to SHRs via oral gavage for 16 weeks. WKY rats were treated with double-distilled water as normotensive controls.
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