Cell viability was assayed in 2D-adherent conditions using 384-well culture plates (Corning, #3570) and ultra-low attachment conditions using PrimeSurface 384-well 3D culture spheroid plates (S-bio, #MS-9384WZ) as previously described.26 (link) In brief, PATU-8988T and PATU-8902 cells were plated at a density of 100 cells per well in 50 μL media and allowed to adhere or form spheroids overnight. Cells were treated with 100 nL of compound from compound stock plates using a Janus Workstation pin tool (PerkinElmer) and incubated for 120 h. Cell viability was measured by addition of 10 μL of CellTiter-glo (Promega), followed by incubation for 15 minutes at room temperature. Luminescence was measured on an EnVision 2104 Multilabel Plate Reader (PerkinElmer). Data was normalized to DMSO-treated wells for each cell line and analyzed using GraphPad PRISM v7.
Protocol detail
Cell Viability Assay in 2D and 3D Cultures
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Dana-Farber Cancer Institute, The University of Texas Southwestern Medical Center, Harvard University, Broad Institute, Beth Israel Deaconess Medical Center, Baylor College of Medicine, Universitat Autònoma de Barcelona, Promega (United States), University of Michigan–Ann Arbor, Korea University, Korea Institute of Science and Technology
Top 5 similar protocols
Protocol cited in 1 other protocol
Variable analysis
independent variables
- Compound treatment
- Cell viability
- Cell lines (PATU-8988T and PATU-8902)
- Cell seeding density (100 cells per well)
- Media volume (50 μL)
- Incubation time (120 h)
- Luminescence measurement (CellTiter-glo and EnVision 2104 Multilabel Plate Reader)
- Data normalization (to DMSO-treated wells)
- DMSO-treated wells (negative control)
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!