For octanoic acid production experiments, strains expressing CpFatB1* under various modes of control (Table S2) were cultured in LB overnight with light illumination to maintain low CpFatB1* expression. Overnight cultures were diluted 1:20 into M9 minimal media with 2% glucose and kept in the light until they reached early stationary phase (OD 600 of 0.6) unless otherwise noted. The LPA was then programmed to either maintain light for low octanoic acid production or turn off light exposure to induce octanoic acid production for 24 hours prior to fatty acid extraction and quantification.
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Octanoic Acid Production Optimization
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Corresponding Organization : Boston University
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Variable analysis
independent variables
- Mode of control for CpFatB1* expression
- Octanoic acid production
- Overnight culture in LB with light illumination
- Dilution of overnight cultures 1:20 in M9 minimal media with 2% glucose
- Early stationary phase (OD 600 of 0.6)
- Light exposure for 24 hours prior to fatty acid extraction and quantification
- Maintain light for low octanoic acid production (negative control)
- Turn off light exposure to induce octanoic acid production (experimental condition)
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