LNCaP and PC3 cells were obtained from the American Type Culture Collection (Manassas, VA). The Ace-1 and Leo cell lines were available from Dr. Rosol's laboratory at The Ohio State University (Columbus, OH) [11 (link),12 (link)]. Mouse monoclonal antibodies 3C6 and 4D8 were obtained from Northwest Biotherapeutics (Seattle, WA). All other chemicals and cell-culture reagents were purchased from Fisher Scientific (Somerville, NJ), Pierce (Rockford, IL), Invitrogen (Grand Island, NY), or Sigma-Aldrich (St. Louis, MO). The PSMA inhibitors CTT-54 and FAMXCTT-54 (Fig. 1 ) were available from prior studies [17 (link),19 (link)].
Protocol detail
PSMA Inhibitors Protocol for Cell Lines
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Corresponding Organization :
Other organizations : Washington State University, The Ohio State University, Cancer Targeted Technology (United States)
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Variable analysis
independent variables
- LNCaP and PC3 cell lines
- Ace-1 and Leo cell lines
- Mouse monoclonal antibodies 3C6 and 4D8
- PSMA inhibitors CTT-54 and FAMXCTT-54
- Measured outcomes (not explicitly mentioned)
- All other chemicals and cell-culture reagents
- Positive controls: Not specified
- Negative controls: Not specified
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