N-glycan purification and pyridylamination were performed as described previously16 (link)17 (link). PA-N-glycans were analyzed by HPLC using the following columns: DEAE (TSKgel DEAE-5PW, Tosoh, Tokyo, Japan), NP (Shodex Asahipak NH2P-50 4E, Showa Denko, Tokyo, Japan) and RP (CAPCELL PAK C18 SG120, Shiseido, Tokyo, Japan or Develosil C30-UG-5, Nomura Chemical, Seto, Japan). Additional details are provided in the Supplementary Information.
Yoshimura T., Hayashi A., Handa-Narumi M., Yagi H., Ohno N., Koike T., Yamaguchi Y., Uchimura K., Kadomatsu K., Sedzik J., Kitamura K., Kato K., Trapp B.D., Baba H, & Ikenaka K. (2017). GlcNAc6ST-1 regulates sulfation of N-glycans and myelination in the peripheral nervous system. Scientific Reports, 7, 42257.
Corresponding Organization : National Institute for Physiological Sciences
Other organizations :
Tokyo University of Pharmacy and Life Sciences, Nagoya City University, The Graduate University for Advanced Studies, SOKENDAI, Nagoya University, Saitama Medical University, Cleveland Clinic Lerner College of Medicine
NP (Shodex Asahipak NH2P-50 4E, Showa Denko, Tokyo, Japan)
RP (CAPCELL PAK C18 SG120, Shiseido, Tokyo, Japan or Develosil C30-UG-5, Nomura Chemical, Seto, Japan)
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