Feeder-free Culture of Human ES Cells

SEES-3 human ES cells were obtained from the National Center for Child Health and Development, Japan [18 (link)], and cultured under feeder-free conditions in StemFit AK02N medium (Ajinomoto, Tokyo, Japan) on laminin511 E8 fragment (iMatrix-511: Nippi, Tokyo, Japan)-coated dishes. All experiments were performed in accordance with the Guidelines for Derivation and Utilization of Human Embryonic Stem Cells by the Ministry of Education, Culture, Sports, Science, and Technology, Japan. Experimental protocols were approved by the Ethics Committee of Keio University School of Medicine for human stem cell experiments (No. 2012-01) and for recombinant DNA experiments (No. 24-010-12).

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Ida H., Akiyama T., Ishiguro K., Goparaju S.K., Nakatake Y., Chikazawa-Nohtomi N., Sato S., Kimura H., Yokoyama Y., Nagino M., Ko M.S, & Ko S.B. (2018). Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors. Stem Cell Research & Therapy, 9, 277.

Publication 2018

StemFit AK02N medium iMatrix-511

Child health Dishes Ethics committee Human Human embryonic stem cells Recombinant dna Sees Stem cell

Corresponding Organization :

Other organizations : Keio University, Nagoya University

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Variable analysis

independent variables

Culture conditions: Feeder-free conditions in StemFit AK02N medium on laminin511 E8 fragment (iMatrix-511)-coated dishes

dependent variables

Not explicitly mentioned

control variables

Cell line: SEES-3 human ES cells obtained from the National Center for Child Health and Development, Japan
Experimental protocols: Approved by the Ethics Committee of Keio University School of Medicine for human stem cell experiments (No. 2012-01) and for recombinant DNA experiments (No. 24-010-12)

controls

Positive control: Not specified
Negative control: Not specified

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