Western Blot Analysis of Phosphorylated Proteins

The expression or activity of the proteins of interest were detected by WB using protein-specific antibody as described previously [9 (link)]. The rabbit anti-phosphorylation p38 MAPK (Thr180/Tyr182), rabbit anti-phosphorylation p44/42 MAPK (Erk1/2) (Thr202/Tyr204) monoclonal antibody and rabbit anti-phosphorylation STAT3 (Phospho-Tyr705) antibody were provided by Cell signaling technologies (Danvers, MA, USA). Mouse anti-p38, rabbit anti-ERK, anti-STAT3 and mouse anti-GADPH and anti-β-actin antibodies were purchased from Beyotime Institute of biotechnology. Rabbit anti-LANA [9 (link)] and anti-RTA [35 (link)] antibodies were prepared by our laboratory.

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Chen J., Jiang L., Lan K, & Chen X. (2015). Celecoxib Inhibits the Lytic Activation of Kaposi’s Sarcoma-Associated Herpesvirus through Down-Regulation of RTA Expression by Inhibiting the Activation of p38 MAPK. Viruses, 7(5), 2268-2287.

Publication 2015

anti-STAT3 anti-β-actin

Actin Anti antibodies Antibodies Antibody Erk1 Lana Monoclonal antibody Mouse P38 mapk Phosphorylation Protein Rabbit Stat3

Corresponding Organization : University of Chinese Academy of Sciences

Other organizations : Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences

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