Quantifying Biomolecules in Cell Samples

Assays of total DNA, protein, and cellular TG content used cell samples lysed and sonicated in the SDS buffer. Total DNA was measured with a fluorescence-based assay using the Hoechst dye method. Total protein content in a sample was determined using a BCA assay kit (Thermo Scientific) per the manufacturer’s instructions. Cellular TG content was measured using an enzymatic assay kit (Sigma Aldrich) as described previously (Si et al. 2009 (link)).

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Manteiga S, & Lee K. (2016). Monoethylhexyl Phthalate Elicits an Inflammatory Response in Adipocytes Characterized by Alterations in Lipid and Cytokine Pathways. Environmental Health Perspectives, 125(4), 615-622.

Publication 2016

BCA assay kit enzymatic assay kit

Assay Buffer Cellular Enzymatic assay Fluorescence Protein Protein a

Corresponding Organization : Tufts University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Total DNA content
Total protein content
Cellular TG content

control variables

None explicitly mentioned

controls

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