Immunohistochemistry of Hepatic Markers

Stainings for CD31 and α-SMA were performed in cryosections from liver tissue (3 and 7 μm thick, respectively) as described previously41 (link)42 (link)43 (link). Briefly, after several steps cryosections were incubated with a mouse-anti-SMA antibody (clone 1A4; Sigma-Aldrich, Munich, Germany), or with antibody against CD31 (ab24590, Abcam, Cambridge, UK). Thereafter, a biotinylated rabbit-anti-mouse secondary antibody (Dako, Glostrup, Denmark) was used.

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Uschner F.E., Ranabhat G., Choi S.S., Granzow M., Klein S., Schierwagen R., Raskopf E., Gautsch S., van der Ven P.F., Fürst D.O., Strassburg C.P., Sauerbruch T., Mae Diehl A, & Trebicka J. (2015). Statins activate the canonical hedgehog-signaling and aggravate non-cirrhotic portal hypertension, but inhibit the non-canonical hedgehog signaling and cirrhotic portal hypertension. Scientific Reports, 5, 14573.

Publication 2015

mouse-anti-SMA antibody (clone 1A4; ab24590 biotinylated rabbit-anti-mouse secondary antibody

Anti antibody Antibody Clone Cryosections Liver Mouse Rabbit Stainings Tissue

Corresponding Organization :

Other organizations : University of Bonn, Duke Medical Center

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Variable analysis

independent variables

Stainings for CD31 and α-SMA

dependent variables

Not explicitly mentioned

control variables

Liver tissue cryosections (3 and 7 μm thick)
Antibodies used (mouse-anti-SMA antibody, antibody against CD31, biotinylated rabbit-anti-mouse secondary antibody)

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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