Huaier Extract Inhibits NLRP3 Inflammasome

Huaier aqueous extract was obtained and prepared as described [24 (link)]. DSS (5000 kDA) was from Wako Pure Chemical Industries(Osaka, Japan); ATP, chloroquine, PMA and LPS (Escherichia coli, 055:B5) were from Sigma-Aldrich(St. Louis, MO, USA); MG132 was from Merck-Millipore(Temecula, CA, USA); 3-MA was from Selleck Chemicals(Houston, TX, USA) ; cycloheximide(CHX) was from ApexBio(Houston, TX, USA); anti-NLRP3 Ab and anti-ASC Ab, anti-caspase-1 p10 Ab and p20 Ab were from Adipogen(San Diego, CA, USA); anti-LC3B Ab was from Cell Signaling Technology(Trask Lane Danvers, MA, USA); anti-IL-1β was from Novus Biologicals(Littleton, CO, USA).