Chemical treatments were adapted from previously described studies (Marra et al., 2019a; Chambers et al., 2020; Wesselman et al., 2023) (link). In short, 16,16-Dimethyl-prostaglandin E2 (Santa Cruz Biotechnology) was dissolved in 100% DMSO (American Bioanalytical) to create 1 M stock. Then 1 M stock was diluted to the treatment dose. Treatments were performed in triplicate with n > 30 embryos per replicate.
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Embryonic Chemical Treatments
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Other organizations : University of Notre Dame
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Variable analysis
independent variables
- 16,16-Dimethyl-prostaglandin E2 concentration
- Embryo response to treatment
- DMSO concentration
- Embryo number per replicate
- Positive control: Not specified
- Negative control: Not specified
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