The plasmids made in this study are listed as follows: pCSIIneo-MCS (multi-cloning site), pCSIIneo-FLAG-BRAF-V600E, pCSIIneo-FLAG-KRAS-G12V, psPAX2 was a gift from Dr. D. Trono, Addgene plasmid #12260) [23 (link)]. pCMV-VSV-G-RSV-Rev (a gift of Dr. Miyoshi, RIKEN, Japan). pCSIIbsr-FLAG-BRAF-V600E was a kind gift from Dr. Matsuda (Kyoto University) [24 (link)].
The antibodies used for western blot and immunofluorescence analyses are as follows: phospho-anti-Erk1/2 (Thr202/Tyr204) (E10) and anti-Erk1/2 (137F5) were from Cell Signaling Technology; anti-KRAS (clone 3B10-2F2) was from Sigma; anti-Raf-B (F-7) and anti-Tubulin (sc-58886) were from Santa Cruz Biotechnology.
The negative control siPOOL, BRAF targeted siPOOL and KRAS targeted siPOOL were purchased from siTOOLs Biotech.
The antibodies used for western blot and immunofluorescence analyses are as follows: phospho-anti-Erk1/2 (Thr202/Tyr204) (E10) and anti-Erk1/2 (137F5) were from Cell Signaling Technology; anti-KRAS (clone 3B10-2F2) was from Sigma; anti-Raf-B (F-7) and anti-Tubulin (sc-58886) were from Santa Cruz Biotechnology.
The negative control siPOOL, BRAF targeted siPOOL and KRAS targeted siPOOL were purchased from siTOOLs Biotech.
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