IHC Staining of Colorectal Tissues

IHC staining of colorectal tissues was performed on 5-µm-thick formalin-fixed and paraffin-embedded (FFPE) tissue sections according to the standard procedures as described previously [14 (link)]. The sections were stained using antibodies against CXCR7 (ab72100) (Abcam, USA), α-SMA (55135-1-AP), Vimentin (10366-1-AP), and Ki67 (27309-1-AP) (Proteintech, USA) according to the manufacturers’ instructions. The IHC results were taken with a digital slide scanning system (Pannoramic Scan, 3DHISTECH Ltd) and semiquantified of mean density (IOD/area) by image-pro plus 6 software (IPP, USA).

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Wang D., Wang X., Song Y., Si M., Sun Y., Liu X., Cui S., Qu X, & Yu X. (2022). Exosomal miR-146a-5p and miR-155-5p promote CXCL12/CXCR7-induced metastasis of colorectal cancer by crosstalk with cancer-associated fibroblasts. Cell Death & Disease, 13(4), 380.

Publication 2022

ab72100) α-SMA (55135-1-AP), Vimentin (10366-1-AP), Ki67 (27309-1-AP) Pannoramic Scan

Antibodies Cxcr7 Embedded paraffin Formalin Scan Staining tissues Taken Tissue Vimentin

Corresponding Organization :

Other organizations : Capital Medical University

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Variable analysis

independent variables

CXCR7 antibody (ab72100)
α-SMA antibody (55135-1-AP)
Vimentin antibody (10366-1-AP)
Ki67 antibody (27309-1-AP)

dependent variables

Mean density (IOD/area) of IHC staining

control variables

Formalin-fixed and paraffin-embedded (FFPE) colorectal tissue sections
Tissue section thickness (5 μm)

Annotations

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