Immunoblotting Assay for TLR and Cadherin Expression

SDS-PAGE and immunoblotting were performed as previously described by Geoghegan et al. [22 (link)]. Antibody information: TLR2 (AF2616-SP), Goat polyclonal (R&D Systems); TLR6 (NBP1-54336), Goat polyclonal (R&D Systems); IL-6Ra (MAB227), Mouse monoclonal IgG₁, Clone 17506 (R&D Systems); gp130 (MAB2281), Mouse monoclonal IgG_1, Clone 29104 (R&D Systems); Vimentin (5741), Rabbit monoclonal, Clone D21H3 XP^® (Cell Signaling); E-cadherin (3195), Rabbit monoclonal, Clone 24E10^® (Cell Signaling); N-cadherin (13116), Rabbit monoclonal, Clone D4R1H XP^® (Cell Signaling); β-catenin (8480), Rabbit monoclonal, Clone D10A8 XP^® (Cell Signaling); β-actin (4970), Rabbit monoclonal (Cell Signaling); GAPDH (CB1001) Rabbit monoclonal (Calbiochem); HRP conjugated anti-goat IgG (705-035-003) (Jackson Immunolabs); HRP conjugated anti-rabbit IgG (W401)(Promega).

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Karavyraki M, & Porter R.K. (2022). Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma. Medical Oncology (Northwood, London, England), 39(6), 60.

Publication 2022

Goat polyclonal Clone 17506 E-cadherin Clone 24E10 N-cadherin β-actin HRP conjugated anti-rabbit IgG

Actin Anti igg Antibody Clone E cadherin Gapdh Goat Gp130 Igg1 Il 6ra Mouse N cadherin Promega Rabbit Sds page Tlr2 Tlr6 Vimentin β catenin

Corresponding Organization :

Other organizations : Trinity College Dublin

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Variable analysis

independent variables

SDS-PAGE and immunoblotting methodology

dependent variables

Levels of TLR2, TLR6, IL-6Ra, gp130, Vimentin, E-cadherin, N-cadherin, β-catenin, β-actin, and GAPDH

control variables

Methodology for SDS-PAGE and immunoblotting as previously described by Geoghegan et al. [22]

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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