Anticoagulation of blood obtained by puncture of the retro-orbital plexus was performed with 0.1 volume of 136 mmol/L trisodium citrate. Subsequently, plasma was immediately isolated by centrifugation at 1100 × g for 10 min and stored at −20 °C [64 (link),67 (link),68 (link)].
Plasma cholesterol and lipoprotein cholesterol levels were determined using a Cholesterol Quantification kit from Sigma-Aldrich (Sigma, St. Louis, MO, USA). HDL and non-HDL lipoproteins were separated by ultracentrifugation as described [69 (link)]. Plasma triglyceride concentration was quantified using the Triglyceride Quantification kit MAK266 (Sigma, St. Louis, MO, USA) according to the instructions of the manufacturer.
Plasma cholesterol and lipoprotein cholesterol levels were determined using a Cholesterol Quantification kit from Sigma-Aldrich (Sigma, St. Louis, MO, USA). HDL and non-HDL lipoproteins were separated by ultracentrifugation as described [69 (link)]. Plasma triglyceride concentration was quantified using the Triglyceride Quantification kit MAK266 (Sigma, St. Louis, MO, USA) according to the instructions of the manufacturer.
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