Immunohistochemical Analysis of Osteopontin and MMPs

The antibodies used were mouse monoclonal anti-OPN (clone OP3N, NOVOCASTRA; Leica Biosystem, Newcastle Upon Tyne, UK) and mouse monoclonal anti-MMP-9 (clone 15W2, NOVOCASTRA; Leica Biosystem, Newcastle Upon Tyne, UK), and mouse monoclonal anti-MMP-2 (clone6E3F8, ABCAM; Biotech, Life Sciences, Cambridge, MA, USA). All antibodies were optimized and validated for IHC in previous studies (Li et al., 2017 (link); Perrotta et al., 2011 (link); Sulik & Chyczewski, 2008 (link)).

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Anbarasen L., Lim J., Rajandram R., Mun K.S, & Sia S.F. (2019). Expression of osteopontin, matrix metalloproteinase-2 and -9 proteins in vascular instability in brain arteriovenous malformation. PeerJ, 7, e7058.

Publication 2019

NOVOCASTRA

Antibodies Clone Mmp 2 Mmp 9 Mouse

Corresponding Organization :

Other organizations : University of Malaya

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Variable analysis

independent variables

Mouse monoclonal anti-OPN (clone OP3N, NOVOCASTRA; Leica Biosystem, Newcastle Upon Tyne, UK)
Mouse monoclonal anti-MMP-9 (clone 15W2, NOVOCASTRA; Leica Biosystem, Newcastle Upon Tyne, UK)
Mouse monoclonal anti-MMP-2 (clone6E3F8, ABCAM; Biotech, Life Sciences, Cambridge, MA, USA)

dependent variables

not explicitly mentioned

control variables

not explicitly mentioned

controls

The antibodies used were optimized and validated for IHC in previous studies (Li et al., 2017; Perrotta et al., 2011; Sulik & Chyczewski, 2008).

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