Balb/3T3 Cells Mitochondrial Membrane Potential Analysis

The Balb/3T3 cells were seeded and treated as described above. Cell culture medium was aspirated out, and the cells incubated with Mito-Tracker Red CMXRos and Hoechst 33,342 in the Mito-Tracker Red CMXRos staining solutions, as described by the manufacturer’s instructions of the Mitochondrial Membrane Potential Kit (# C1071S, Beyotime) at room temperature in the dark for 20–30 min. The contents were immediately place in an ice bath, then subjected to inverted fluorescence microscopy for detection of cell fluorescence. Mito-Tracker Red CMXRos and Hoechst 33,342 were detected as red and blue fluorescence, respectively [21 (link)].