Twenty thousand CaSki cells per well were seeded in a 12-well plate. Drugs were added at 24 h after doxycycline induction. At 72 h after drug treatment, cells were incubated with fresh medium containing 10 μg/ml resazurin (Sigma). resazurin conversion was measured with a Synergy H1 microplate reader (BioTek) with 560-nm excitation and 590-nm emission filters as previously described (80 (link)).
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