Example 6
Round 3-D inserts (having three micro-channels of 3 mm width by 20 mm length by 10 mm height) were seeded with 10,000 P6 cells human bone marrow-derived mesenchymal stem cells (labeled with cell tracker red) per channel in 200 μl of culture media in a tissue culture dish on a rocking platform. Rocking frequency was 5 rpm and 15° tilt. After one-day culture, another 10,000 cells (labeled with cell tracker green) were added to the same channel and cultured for another day.
It should be understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application and the scope of the appended claims. In addition, any elements or limitations of any invention or embodiment thereof disclosed herein can be combined with any and/or all other elements or limitations (individually or in any combination) or any other invention or embodiment thereof disclosed herein, and all such combinations are contemplated with the scope of the invention without limitation thereto.
