Lymphocyte Subset Quantification by Flow Cytometry

EDTA anticoagulated peripheral blood as flow cytometry samples was drawn from the participants before initial treatment to determine lymphocyte and subsets. All samples were tested within 6 hours of being obtained. Briefly, CD3+/CD4+/CD8+ T-cell, CD19+ B-cell, and CD16+ CD56+ NK-cell counts (cells/μL) were measured by multiple-color flow cytometry with human monoclonal anti-CD3-fluorescein isothiocyanate (FITC), anti-CD4-phycoerythrin (PE), antiCD8-allophycocyanin (APC), anti-CD19-PE, and anti-CD56-PE antibodies (BD Multitest) according to the manufacturer’s instructions. The cells were analyzed on a BD FACS Canto II flow cytometry system (BD Biosciences) (15 (link), 19 (link)).

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Liu D., Yuan X., Gao F., Zhao B., Ding L., Huan M., Liu C, & Jiang L. (2022). High Number and Specific Comorbidities Could Impact the Immune Response in COVID-19 Patients. Frontiers in Immunology, 13, 899930.

Publication 2022

anti-CD4-phycoerythrin antiCD8-allophycocyanin anti-CD19-PE anti-CD56-PE antibodies BD FACS Canto II

Allophycocyanin Anti antibodies Anti cd3 B cell Blood flow Cd4 t cell Cells Edta Flow cytometry Fluorescein Human Isothiocyanate Lymphocyte Phycoerythrin

Corresponding Organization : Public Health Clinical Center of Chengdu

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Variable analysis

independent variables

No independent variables were explicitly mentioned in the protocol.

dependent variables

Lymphocyte and subset counts (cells/μL) for CD3+/CD4+/CD8+ T-cells, CD19+ B-cells, and CD16+ CD56+ NK-cells

control variables

Time of sample testing (all samples were tested within 6 hours of being obtained)

controls

No positive or negative controls were explicitly mentioned in the protocol.

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