Electrophysiological Analysis of Parkinson's Disease Neurons

Control iCell DopaNeurons (Fujifilm Cellular Dynamics Inc.) and MyCell SNCA A53T DopaNeurons were thawed and dotted (~80k cells) onto a 48-well MEA plate (Axion Biosystems) using the iCell DopaNeuron MEA application protocol. Cultures were treated with BrainPhys medium (Stem Cell Technologies), with 50% medium changes performed every two to three days for 20 days. Five-minute recordings were made on DIV20 post-plating (N = 24). Raw voltage recordings were processed via a Butterworth (200–4 kHz) filter; action potentials were then detected by a 5.5 standard deviation detection threshold, and network-level bursting behaviors were analyzed off-line using the Axion’s NeuralMetric toolbox via the ‘Envelope’ algorithm (threshold factor 3, minimum inter-burst-interval 10 s, burst inclusion percentage 75, minimum number of electrodes percentage 25) and synchrony index (20 ms)^{48 (link)}. The activity and network-level bursting behaviors assessed included mean firing rate, network bursting rate (bursts per minute: BPM), intensity (Hz), and duration (seconds). A two-tailed student t-test was used to assess statistical differences on each measure independently between the two groups of neurons.

Free full text: Click here

Stern S., Lau S., Manole A., Rosh I., Percia M.M., Ben Ezer R., Shokhirev M.N., Qiu F., Schafer S., Mansour A.A., Mangan K.P., Stern T., Ofer P., Stern Y., Diniz Mendes A.P., Djamus J., Moore L.R., Nayak R., Laufer S.H., Aicher A., Rhee A., Wong T.L., Nguyen T., Linker S.B., Winner B., Freitas B.C., Jones E., Sagi I., Bardy C., Brice A., Winkler J., Marchetto M.C, & Gage F.H. (2022). Reduced synaptic activity and dysregulated extracellular matrix pathways in midbrain neurons from Parkinson’s disease patients. NPJ Parkinson's Disease, 8, 103.

Publication 2022

BrainPhys medium

Action potentials Cells Mea protocol Neurons Seconds Snca Stem cell Student

Corresponding Organization :

Other organizations : Salk Institute for Biological Studies, University of Haifa, Technical University of Munich, Hebrew University of Jerusalem, Cellular Dynamics International (United States), Fujifilm (United States), Weizmann Institute of Science, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, South Australian Health and Medical Research Institute, Flinders University, Institut du Cerveau, University of California, San Diego

Top 5 similar protocols

Variable analysis

independent variables

Treatment with BrainPhys medium

dependent variables

Mean firing rate
Network bursting rate (bursts per minute: BPM)
Burst intensity (Hz)
Burst duration (seconds)
Synchrony index (20 ms)

control variables

Cell type (iCell DopaNeurons and MyCell SNCA A53T DopaNeurons)
Culture duration (20 days post-plating)
Recording duration (5 minutes)
Electrode type (48-well MEA plate)
Data processing (Butterworth 200–4 kHz filter, action potential detection threshold, network-level bursting analysis parameters)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!