Quantification of LncRNA, mRNA, and miRNA

Trizol (Invitrogen, CA, USA) was utilized for extracting total RNA in myocardial tissues. The concentration and quality of RNA were determined by NanoDrop2000 (Thermo Fisher Scientific, MA, USA). Expression of LncRNA and mRNA was quantified using PrimeScript™ RT reagent Kit (TaKaRa, Dalian, China)  and TB Green™Premix Ex Taq™ II (TaKaRa). miRNA expression was calculated using miRcute Plus miRNA First-Strand cDNA Kit and miRcute Plus miRNA qPCR Kit (SYBR Green) (Tiangen) (Fu et al. 2020 (link)). The primers were composed by BGI Co. (Shenzhen, Guangdong, China) (Additional file 3: Table S1). U6 was the endogenous control of miR-185-3p while glyceraldehyde phosphate dehydrogenase (GAPDH) was that of LINC00461 and Myd88. Data were reckoned by 2^−ΔΔCt method (Livak and Schmittgen 2001 (link)).