All blood samples were drawn after a 12-hour fast. Serum oxLDL was measured by enzyme-linked immunosorbent assay (ELISA) (Mercodia, Uppsala, Sweden). The intra and inter-assay variability were 6.3% and 4.7%, respectively. Soluble CD163 (Trillium Diagnostics, intra-assay variability 3–6% and inter-assay variability 5–8%)) and soluble CD14 (R&D Systems, intra-assay variability 4.8–6.4% and inter-assay variability 4.8–7.4%) were measured by ELISA. Direct LDL, total cholesterol, high-density lipoprotein (HDL), triglycerides, glucose, hemoglobin A1c, and lipoprotein-associated phospholipase A2 (Lp-PLA2) were measured as previously described [19 (link)].
Protocol detail
Serum Biomarkers in Cardiovascular Disease
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Assay
Blood
Cd163
Cholesterol
Diagnostics
Enzyme linked immunosorbent assay
Glucose
Hemoglobin a1c
High density lipoprotein
Lipoprotein associated phospholipase a2
Oxldl
Serum
Soluble cd14
Triglycerides
Trillium
Corresponding Organization : Massachusetts General Hospital
Other organizations : October 6 University
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Variable analysis
independent variables
- None explicitly mentioned
- Serum oxLDL
- Soluble CD163
- Soluble CD14
- Direct LDL
- Total cholesterol
- High-density lipoprotein (HDL)
- Triglycerides
- Glucose
- Hemoglobin A1c
- Lipoprotein-associated phospholipase A2 (Lp-PLA2)
- 12-hour fast prior to blood sample collection
- No positive or negative controls were explicitly mentioned.
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