Conversion of anhydride to acid was performed by hydrolysis in NaOH. Here 800 mg of polymer was dissolved in 2 g of KOH, 20 mL of water in a 100 mL round botom and reflux the mixture for 4 h. This initially dissolved the polymer and at the end, reaction mixture becomes clear. The hydrolyzed polymer was subjected to dialysis using 3.5 KDa cut off membrane for 36 h. After that the polymer was precipitated by the addition of water and HCl(aq). Centrifuging the precipitate was followed by decanting off the supernatant. Wash the precipitate using 0.1 N HCl water three times and dry the precipitate using nitrogen. A brownish solid polymer material was collected.
Protocol detail
Hydrolytic Conversion of Anhydride Polymers
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Corresponding Organization :
Other organizations : Yale University, Research Network (United States), Boston Children's Hospital, Harvard University, Yale Cancer Center
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Variable analysis
independent variables
- Concentration of NaOH
- Reaction time
- Conversion of anhydride to acid
- Characteristics of the hydrolyzed polymer (e.g., molecular weight, solubility, purity)
- Mass of polymer (800 mg)
- Concentration of KOH (2 g in 20 mL of water)
- Dialysis conditions (3.5 kDa cutoff membrane, 36 h)
- Precipitation conditions (addition of water and HCl(aq))
- Washing conditions (0.1 N HCl, 3 times)
- Positive control: Not mentioned
- Negative control: Not mentioned
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