Cells were transfected with arrestin3-GFP along with D2Arr or D2G. Cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) for 10 min at 20°C. The cells were subsequently mounted on slides using Vectashield (Vector Laboratories; Burlingame, CA, USA) and imaged using a laser scanning confocal microscope (TCS SP5/AOBS/Tandem; Leica, Jena, Germany). The images were analyzed using the Fiji version of the image processing software ImageJ and colocalization was analyzed based on Pearson’s correlation coefficient (γ value) (Min et al., 2023 (link)).
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Imaging Arrestin3-GFP Colocalization
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Other organizations : Chonnam National University
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Variable analysis
independent variables
- Arrestin3-GFP
- D2Arr
- Colocalization of arrestin3-GFP with D2Arr or D2G, as analyzed by Pearson's correlation coefficient (γ value)
- Cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) for 10 min at 20°C
- Cells were mounted on slides using Vectashield (Vector Laboratories; Burlingame, CA, USA)
- Cells were imaged using a laser scanning confocal microscope (TCS SP5/AOBS/Tandem; Leica, Jena, Germany)
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