Curcuminoid Separation via HPLC Gradient Elution

Separation of the curcuminoids was carried out using gradient elution. The mobile phase consisted of two eluents: A, 0.1% acetic acid in acetonitrile (Fisher Scientific, Pittsburgh, PA), and B, 0.1% acetic acid in HPLC-grade water (Fisher). The eluent composition was varied linearly from 50% to 100% A over the period 0 to 10 min with a plateau at 100% A from 10 to 11 min. The composition returned to 50% A over the period 11 to 12 min followed by a reconditioning at 50% A from 12 to 20 min. The flow rate was 0.5 ml/min, and the column was a Phenomenex Aqua (Phenomenex, Torrance, CA) endcapped C₁₈ having dimensions 150 × 4.6 mm with a 5-μm particle size. The column was heated at 40°C, and the samples were stored at room temperature in the autosampler. The total analysis time was 20 min. A volume of 25 μl was injected. Retention times for the two monitored curcuminoid peaks were 6.4 and 9.6 min.

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Lee J.C., Kinniry P.A., Arguiri E., Serota M., Kanterakis S., Chatterjee S., Solomides C.C., Javvadi P., Koumenis C., Cengel K.A, & Christofidou-Solomidou M. (2010). Dietary Curcumin Increases Antioxidant Defenses in Lung, Ameliorates Radiation-Induced Pulmonary Fibrosis, and Improves Survival in Mice. Radiation research, 173(5), 590-601.

Publication 2010

Acetic acid Acetonitrile Curcuminoid Hplc Retention

Corresponding Organization :

Other organizations : University of Pennsylvania, Temple University

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Variable analysis

independent variables

Gradient elution of the mobile phase, with the eluent composition varying linearly from 50% to 100% A over 0 to 10 min, followed by a plateau at 100% A from 10 to 11 min, and then returning to 50% A over 11 to 12 min

dependent variables

Retention times for the two monitored curcuminoid peaks (6.4 and 9.6 min)

control variables

The mobile phase consisted of two eluents: A, 0.1% acetic acid in acetonitrile, and B, 0.1% acetic acid in HPLC-grade water
The flow rate was 0.5 ml/min
The column was a Phenomenex Aqua endcapped C18 (150 × 4.6 mm, 5-μm particle size)
The column was heated at 40°C
The samples were stored at room temperature in the autosampler
The total analysis time was 20 min
The injection volume was 25 μl

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