Zebrafish Genotypes and Metamorphosis

We investigated zebrafish (Danio rerio) of the following genotypes: nac^w2, pfe^tm236, cho^tm26 (ZFIN database), shd^j9s1 (Lopes et al., 2008 (link)) and Tg(TDL358:gfp) (Levesque et al., 2013 (link)). We describe two new n-ethyl-n-nitrosourea (ENU)-induced rse alleles, rse^tAN17X (weak) and rse^tLF802 (strong), which we will refer to as rse^w and rse^s or simply rse. The phenotype of the stronger allele resembles that of the published amorphic allele (Parichy et al., 2000b (link)). Zebrafish were maintained as described by Brand et al. (Brand et al., 2002 ). Metamorphic fish were staged [AR, anal fin ray/6.2 mm standardised standard length (SSL); PB, pelvic fin bud/7.2 mm SSL; PR, pelvic fin ray/8.6 mm SSL; SP, squamation onset posterior/9.6 mm SSL; J, juvenile/11.0 mm SSL; J+, juvenile+/13 mm SSL; and J++, juvenile++/16 mm SSL] according to Parichy et al. (Parichy et al., 2009 (link)).

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Frohnhöfer H.G., Krauss J., Maischein H.M, & Nüsslein-Volhard C. (2013). Iridophores and their interactions with other chromatophores are required for stripe formation in zebrafish. Development (Cambridge, England), 140(14), 2997-3007.

Publication 2013

Allele Anal Fish Genotypes Nitrosourea Pelvic Phenotype Weak Zebrafish

Corresponding Organization :

Other organizations : Max Planck Institute for Developmental Biology

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Variable analysis

independent variables

Genotypes: nac^w2, pfe^tm236, cho^tm26, shd^j9s1, Tg(TDL358:gfp)
Rse alleles: rse^w (weak) and rse^s (strong)

dependent variables

Phenotype of the stronger rse allele (rse^s)

control variables

Zebrafish were maintained as described by Brand et al. (2002)
Metamorphic fish were staged according to Parichy et al. (2009)

controls

Positive control: Published amorphic allele (Parichy et al., 2000b)
Negative control: Not explicitly mentioned

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