The scaffold porosity and the distribution of the β-TCP particles in the scaffold were detected using a Quantum GX2 micro-CT imaging system (PerkinElmer, Hopkinton, MA, USA) at a voxel size of 72 µm. Typically, three samples randomly selected were placed on a 35 mm diameter object bed. A complete scan (scanning parameters: energy = 90 kV; intensity = 88 µA) was then performed. The scanned microstructural images were reconstructed using Invesalius 3.1 software (©2007–2017, Center for Information Technology Renato Archer CTI). The total porosity, pore size, and open porosity of each scaffold were analyzed. In order to obtain the total porosity p, the scaffold volume VS was compared to the theoretical volume of the cylinder VT using Equation (1) [10 (link)]:
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