Cultivation and Maintenance of T. vaginalis

T. vaginalis strain G3, the genome reference strain commonly used in research, isolated from Kent, United Kingdom, in 1963, was used for all experiments in this study (15 (link), 74 (link)). Parasites were cultured in modified Diamond’s medium (75 (link)) supplemented with 10% horse serum, penicillin and streptomycin (Invitrogen), and iron solution composed of ferrous ammonium sulfate and sulfosalicylic acid (Fisher Scientific), as described previously (76 (link)).

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Warring S.D., Blow F., Avecilla G., Orosco J.C., Sullivan S.A, & Carlton J.M. (2021). Small RNAs Are Implicated in Regulation of Gene and Transposable Element Expression in the Protist Trichomonas vaginalis. mSphere, 6(1), e01061-20.

Publication 2021

penicillin and streptomycin sulfosalicylic acid

Cultured medium Diamond Ferrous ammonium sulfate Genome Horse Iron Parasites Penicillin Serum Strain Streptomycin Sulfosalicylic acid

Corresponding Organization :

Other organizations : New York University

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Variable analysis

independent variables

T. vaginalis strain G3, the genome reference strain commonly used in research

dependent variables

Not explicitly mentioned

control variables

Modified Diamond's medium supplemented with 10% horse serum, penicillin and streptomycin (Invitrogen), and iron solution composed of ferrous ammonium sulfate and sulfosalicylic acid (Fisher Scientific)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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