Lentiviral transfection of HCC cells was performed using mRFP-GFP-LC3 (GeneChem Co., Ltd., Shanghai, China), according to the manufacturer’s instructions. Following puromycin selection for 2 weeks, images were captured by confocal fluorescence microscopy (Carl Zeiss, Oberkochen, Germany). Yellow or red fluorescence was quantified to monitor the progression of autophagic flux under normoxia or hypoxia. The number of puncta per cell was counted in five random images. TEM was performed as previously described.11 (link)
Li Q., Ni Y., Zhang L., Jiang R., Xu J., Yang H., Hu Y., Qiu J., Pu L., Tang J, & Wang X. (2021). HIF-1α-induced expression of m6A reader YTHDF1 drives hypoxia-induced autophagy and malignancy of hepatocellular carcinoma by promoting ATG2A and ATG14 translation. Signal Transduction and Targeted Therapy, 6, 76.
Corresponding Organization : Jiangsu Province Hospital
Other organizations :
Chinese Academy of Medical Sciences & Peking Union Medical College, Academia Sinica, Academy of Medical Sciences, Shenzhen Second People's Hospital, Nanjing Drum Tower Hospital, Southeast University
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