Melanomas were sequenced from snap-frozen tumors (Yale and CSU cohorts) or low passage cell cultures (<4) as previously described2 (link),4 (link) (Supplementary Data 1, 8). DNA from melanoma cells and freshly frozen tumors was extracted with the DNeasy purification kit (Qiagen Inc., Valencia, CA). High melanin content was removed with OneStep™ PCR Inhibitor Removal Kit (Zymo Research Corporation, Irvine, CA). Direct-zol™ RNA MiniPrep w/ Zymo-Spin™ IIC Columns (Zymo catalog no. D4019) were used to extract RNA from tumors, and the RNeasy PowerLyzer Tissue & Cells Kit (Qiagen, catalog no. 15055-50) was used to extract RNA from peripheral blood mononuclear cells (PBMCs) and melanoma cells.
Farshidfar F., Rhrissorrakrai K., Levovitz C., Peng C., Knight J., Bacchiocchi A., Su J., Yin M., Sznol M., Ariyan S., Clune J., Olino K., Parida L., Nikolaus J., Zhang M., Zhao S., Wang Y., Huang G., Wan M., Li X., Cao J., Yan Q., Chen X., Newman A.M, & Halaban R. (2022). Integrative molecular and clinical profiling of acral melanoma links focal amplification of 22q11.21 to metastasis. Nature Communications, 13, 898.
Other organizations :
California Institute for Regenerative Medicine, Stanford University, IBM (United States), Xiangya Hospital Central South University, Central South University, Yale University, Chinese Academy of Medical Sciences Dermatology Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Hunan Cancer Hospital, Third Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
