As mentioned previously (35 (link)), macroscopic and microscopic examinations were performed to define the types of organisms and check for blood and mucus. Stool samples were stained with Wheatley Trichrome for Blastocystis detection, following the manufacturer’s instructions. Then, smears were examined microscopically under ×40 and ×100 magnification objectives. Two microbiologists examined all stool slides independently.
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