We infected MDCK or MDCK-SIAT1 cell monolayers in 96-well plates with 50 μl/well of serial dilutions of the virus in the maintenance medium without trypsin. After 1 h incubation at 35°C in 5% CO2, we removed the virus, added 100 μl/well of either MC or Avicel overlay media, and incubated the cells for further 24 h to allow plaque formation. Fixation and immuno-staining were performed as described above for 6-well plates but using 50 μl of reagents per well.
Free full text: Click here
