Fluorochrome-conjugated monoclonal antibodies to mouse CD4, KJ1.26, IL-4, and Foxp3 were obtained from Biolegned. IL-4 ELISA kit was purchased from Biolegend. The lunasin peptide with 43-amino acid was chemically synthesized with 97% purity by LifeTein (South Plainfield, NJ) [16 (link)]. Ovalbumin (OVA, chromatographically purified) was from Worthington Biochemical Corp (Lakewood, NJ).
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